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biorex column  (Bio-Rad)


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    Bio-Rad biorex column
    Biorex Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biorex column/product/Bio-Rad
    Average 94 stars, based on 218 article reviews
    biorex column - by Bioz Stars, 2026-03
    94/100 stars

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    Purification of Heteractis crispa toxins. ( a ) Ion-exchange chromatography of the peptide fraction obtained after hydrophobic chromatography was carried out on Bio-Rex 70 column (2.5 × 60 cm); ( b ) Ion-exchange chromatography of fraction 2 was carried out on SP-Sephadex C-25 column (2.5 × 50 cm). ( c – e ) RP-HPLC of fractions 1 and 3 on Luna C18 (10 × 250 mm) column. Insets: ESI mass spectra, average molecular masses of peptides are shown. Collected fractions are accentuated by solid lines.

    Journal: Toxins

    Article Title: New Insights into the Type II Toxins from the Sea Anemone Heteractis crispa

    doi: 10.3390/toxins12010044

    Figure Lengend Snippet: Purification of Heteractis crispa toxins. ( a ) Ion-exchange chromatography of the peptide fraction obtained after hydrophobic chromatography was carried out on Bio-Rex 70 column (2.5 × 60 cm); ( b ) Ion-exchange chromatography of fraction 2 was carried out on SP-Sephadex C-25 column (2.5 × 50 cm). ( c – e ) RP-HPLC of fractions 1 and 3 on Luna C18 (10 × 250 mm) column. Insets: ESI mass spectra, average molecular masses of peptides are shown. Collected fractions are accentuated by solid lines.

    Article Snippet: The hydrophobic 20% aqueous-ethanolic fraction was further separated by cation-exchange chromatography on Bio-Rex 70 column (2.5 × 60 cm) (Bio-Rad, Richmodn, CA, USA) equilibrated with an ammonium acetate buffer (pH 4.5) at a flow rate of 22 mL/60 min and a fraction size of 5.8 mL, followed by cation-exchange chromatography on Sephadex C-25 (2.5 × 40 cm) (Amersham Biosciences, Piscataway, NJ, USA) at a flow rate of 70 mL/60 min and a fraction size of 7 mL.

    Techniques: Purification, Ion Exchange Chromatography, Chromatography